Indexus TriSense Dx Instrument 
  • 3 Diverse Application Categories
  • Expandable Test Menu
  • 1 Easy-to-Use Instrument

Indexus TriSense Dx is a multifunction instrument platform that can operate both as an automated hematology analyzer delivering CBC results with 5-part differential and as a sensitive multi-color fluorescence flow cytometer performing cellular and bead-based  molecular Dx and immunoassays. It is a compact, low-cost, benchtop device that is easily transportable. The instrument is designed for ease-of-use in small and medium sized laboratories. Using the graphical user interface (GUI) of the instrument, the operator will select to run the instrument either in an automated hematology analyzer mode or in a flow cytometer mode, depending on the test prescribed by the physician for any given sample.


Preliminary Hardware Specifications:

Dimensions: 1' x 1' x 1.25'


Weight: Less than 25 lbs.


Power: 110/220 V AC


Wireless Connectivity:Yes 

Detection: 3 Light Scatter Channels, 2 Fluorescence Channels (expandable to four channels)


Illumination: Single Solid-State Laser


Data Acquisition: 8-Channel High Speed Digital Data Acquisition System



Trisense Combo Graphics 2020.jpg.png
  • Detects DNA, RNA, antibodies and antigens
  • Identifies cells based on morphology and/or immunophenotypes 
Sensitive laser fluorescence sensing technology
High resolution multi-angle light scatter analysis
Switchable modes for Hematology and Flow Cytometry
Compact benchtop instrument

Application Specifications:  

​Automated Complete Blood Count with 5-Part Differential
Immature granulocytes
Light Scatter based lymphocyte subset analysis
Cellular immunophenotyping by fluorescence
CD4 Counts
Bead-based fluorescence immunoassays for protein biomarkers
  • Fully Automated hematology (on-board and in-line sample preparation)

  • Automated flow cytometry fluidics similar to other gold-standard standard commercials platforms where off line sample preparation is required

  • Hematology and Flow Cytometry Fluidics are integrated, and each of these operation mode are user selected on GUI

  • 8 single-ended analog inputs with a maximum signal level of 1.5v to 6v.

    • Programmable gain provides flexibility in detector selection and configuration.

    • Programmable signal offset is used to remove small fixed signal offsets.

    • Baseline restoration is used to dynamically remove slowly fluctuating signal offsets.

    • Programmable bandwidth to optimally condition the signals before digitization.

  • Supports transit times from 2 microseconds to over 10 milliseconds.

  • Collect data up to 1,500 events per second with 8 detectors.

    • Collect even faster with fewer detectors.

  • LVTTL trigger output for triggering other instrumentation when particles are detected.

  • Supports PMTs with built-in high voltage power supplies.

  • Save list mode data in FCS 3.0 format, or correlated raw waveforms in CSV format.

    • List mode data includes Peak and Area from all detectors, plus Width and Time

Key Performance Criteria (Prototype Results)
Fluorescence Sensitivity and Resolution
Data showing Bangs Lab 8-Peak fluorescence standards beads on the FL2-H channel of the TriSense Dx platform
Light-scatter Sensitivity and Resolution
Data showing Bangs Lab light scatter beads (mix of diameters 0.5 and 2.5 microns) measured on SSC and ALL channels
Hematology 5-part White Blood Cell Differential
(on the Indexus TriSense Dx platform)
RBC and Platelet Analysis
(on Indexus TriSense Dx)
CD4 and CD8 lymphocyte subset analysis performed on the Indexus TriSense Dx platform
Measurement of HIV antibody using bead-based immunoassay (on the Indexus TriSense Dx platform). Here various titrated amounts of HIV antinbodies were detected in human serum matrix.
HIV-antibody test results for patient samples run on the Indexus TriSense Dx platform, uisng bead-based immunoassay. Five of the samples were HIV positive (red) and five HIV-negative (black). Results were 100% accurate.
Measurement of HIVp24 protein using bead-based immunoassay on the Indexus TriSense Dx platform. Here titrated amounts of p24 antigens were detected in human serum matrix. The black histogram is a serum sample with no p24 added in the serum, and the colored histograms represent serum samples with p24.