Leveraging fluorescence flow cytometry capabilities on the TriSense Dx Multifunction platform to bring necessary immunoassay test menu without having to purchase expensive immunoassay instruments
Bead-based immunoassay protocols have been used to perform singleplex and multiplex IVD tests. In the industry often this protocol is referred to as Luminex assay, named after an industry leader of the same name. However, due to the complexity and high-cost of commercial flow cytometers, this immunoassay method is not widely accessible to the small and medium sized diagnostic laboratories, even though reagents for various assays are readily available from many reputable vendors.
The fluorescence detection capabilities built into our TriSense Dx platform for CD4 count and other cellular immunoassays have been successfully tested also for the performance of bead-based immunoassays. The figures below describe the basics of bead-based assay principles and demonstrate an example of its application on the Indexus H5F platform, performing the HIV antibody assay. The same format can be applied to any antibody based assay, as well as antigen assays.
Figure 1. Principle of bead-based HIV-antibody assay configuration
Figure 2. Results showing the detection of different titrated amounts of HIV antibody in human serum on the Indexus TriSense Dx platform.
Figure 3. Results for ten patient samples run on the Indexus TriSense Dx platform uisng bead-based immunoassay. Five of the samples were HIV positive and five HIV-negative. Results were 100% accurate.