CD4 and CD8 Count Method Comparison
IndexusTriSense Dx System versus Becton Dickinson® FACSCalibur Flow Cytometer 
 
We measured CD4 andCD8 counts in human blood samples on the Indexus TriSense Dx multifunction platform and compared our results with those obtained on a Becton Dickinson® FACSCalibur flow cytometer. The measurements on the FACSCalibur were performed at Duke University Human Vaccine Institute by Duke University staff, utilizing Becton Dickinson® CD4 and CD8 reagent kit. Indexus utilized reagents procured from a different vendor. 
CD4 cells were identified on the Indexus TriSense Dx instrument by simultaneously labeling the cells with FITC conjugated anti-CD4 antibodies and PE conjugated anti-CD3 antibodies. In the bivariate plot below, the green dots represent the CD4 positive T-cells.  
The graphs above show the comparison of our CD4 count results on ten whole blood samples, five of which were HIV positive (red dots) and five HIV negative (blue dots). For both CD4 percent and absolute counts, comparisons were made for percent counts and absolute number per microliter. 
 
CD4
CD8 cells were identified on the Indexus TriSense Dx instrument by simultaneously labeling the cells with FITC conjugated anti-CD8 antibodies and PE conjugated anti-CD3 antibodies. In the bivariate plot below, the green dots represent the CD8 positive T-cells.  
The graphs above show the comparison of our CD8 count results on ten whole blood samples, five of which were HIV positive (red dots) and five HIV negative (blue dots). For both CD8 percent and absolute counts, comparisons were made for percent counts and absolute number per microliter. 
 
CD8
 
CD4 COUNT: A SUMMARY
  • A CD4 count ranges from 500–1,200 cells/mm3  in healthy adults/adolescents.
  • A CD4 count of fewer than 200 cells/mm3 is one of the qualifications for a diagnosis of stage 3 infection (AIDS).
  • ART is recommended for everyone with HIV, but the urgency to start ART is greater in people with low or rapidly falling CD4 counts.
  • CD4 count can vary from day to day. It can also vary depending on the time of day  blood is drawn and on whether the patient has other infections or illnesses, like the flu or STIs.
  • Typically, the HIV care provider will check a patient's CD4 count every 3 to 6 months when starting ART. Once the CD4 level rebounds and the viral load is suppressed, the provider may check CD4 count less frequently.
 
          source: www.aids.gov